In 2000, Production I.G. and Sony Computer Entertainment Japan co-produced a two-volume Blood: The Last Vampire video game. The game features a musical score by Yuki Kajiura with Youki Kudoh reprising her role as the voice of Saya, and over two hours of theater quality animation. It is a graphical adventure that brings Saya and her hunt for Chiropterans to Tokyo in 2000. There she meets a seventeen-year-old boy who begins wondering about Saya and the history of "Blood". Both volumes of the game were released to the PlayStation 2 in Japan on December 21, 2000. Famitsu magazine scored the first volume a 33 out of 40. Animerica's Dr. Brown called the original game "boring", but did compliment it for having "beautifully animated sequences".
In 2006, Production I.G and Sony re-released the game. Both volumes were combined into a single game for the PlayStation Portable (PSP). The game was released in Japan on January 26, 2006. The combined game included new cover art and additional features, including a strategy flow chart, a digital art gallery, and some exclusive films.